This project is a collection of squamate and amphibian specimens scanned at the University of Colorado Materials Instrumentation and Multimodal Imaging Core (MIMIC) Facility in collaboration with the University of Colorado Museum of Natural History (UCM) and funded by the US National Science Foundation (NSF). The oMeso project is an NSF Partner to an Existing Network (PEN) award to the University of Colorado Museum of Natural History to join the openVertebrate (oVert) Thematic Content Network. oMeso employs 2D and 3D specimen imaging technologies to facilitate exploration of structural and functional diversity in Mesoamerican reptiles and amphibians. The unique ecology and geology of Mesoamerica has fostered the rapid evolution of multiple species adapted for a variety of habitats and life modes. Computed tomography (CT) scans of internal anatomy paired with high resolution photos of external traits provide complimentary image datasets to create an accessible resource for one of the world’s most hyperdiverse regions.See linked occurance records to view 2D images hosted on the iDigBio portal, or the oMeso Project page in Arctos: https://arctos.database.museum/project/10003438.